Genetic Screening in Model Organisms

Using gene silencing (RNAi) in the nemotode C. elegans, students will identify genetic modifiers of proteins with roles in aging by reverse genetics. Analyzing the effect of knocking down genes on the level of aging-related proteins tagged with fluorophores (GFP, RFP, etc.). Students will use function-specific RNAi libraries (transcription factors, kinases, etc) established in our lab. Students will evaluate the effect of genetic modifiers on proteostasis and lifespan, also familiarize C. elegans work and appreciating the use of model organisms, the students will master microscopy, genetic crosses, gene silencing, and molecular and biochemical readout assays such as qPCR and immunoblotting. Expected: BIOL 0200 or equivalent.