Biotechnology Laboratory: Molecular Biology Basics

1 Unit

This course provides basic hands-on laboratory experience in molecular biology with a focus on handling and manipulating DNA in bacterial systems. Specific topics include: General laboratory safety, buffers, media, and other reagent preparation, sterile technique, transformation and culture of bacterial cells, DNA molecular biology techniques including DNA isolation and purification, polymerase chain reaction (PCR), restriction digests, ligation, agarose gel electrophoresis, and sequence analysis. Prereq: Biochemistry Graduate student or Requisites Not Met permission.