Biochemical and Cellular Techniques for Biotechnology

3 Units

This lecture course covers the basics of common, essential laboratory and analytical techniques used in biomedical research and the biotechnology industry. The course will cover recombinant protein production and characterization, mammalian cell culture, molecular and cell biology, and mass spectrometry. Specific topics include: general laboratory safety, record keeping, preparation of research reports, manipulation of bacteria, protein overexpression and purification, enzyme assays, high-throughput techniques, high performance liquid chromatography (HPLC) and mass spectrometry, mammalian cell culture, Western blotting, protein-protein interactions, reverse transcription-quantitative polymerase chain reaction (RT-qPCR), immunofluorescence microscopy and assays for gene expression. This course is suitable for Biochemistry MS students interested in pursuing careers in academia or biotechnology. It is also recommended for undergraduate students to enhance their technical skills and position them for productive research experiences. Graduate students in other programs within or outside the School of Medicine are permitted to enroll. Prereq: ( BIOL 215L and CHEM 113 ) or Graduate standing. Coreq: CHEM 233 or Graduate standing.