Biotechnology Laboratory - CRISPR

2 Units

This course provides advanced hands-on laboratory experience in molecular biology, with a focus on using CRISPR as a tool to manipulate eukaryotic systems. Students will master the laboratory techniques involved in the establishment, verification, and analysis of a CRISPR-edited eukaryotic system, as well as learn the theory behind designing such systems. Topics include: molecular cloning and plasmid preparation; human cell culture, transfection, and DNA extraction; fluorescence microscopy; PCR analysis and guide RNA design. Prereq: BIOC 502B .