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CHEM 477

Advanced Laboratory in Biochemistry

3 credits · Undergraduate

Fall 2021

Topics

  • Protein structure and modelling
  • Enzyme kinetics
  • Chemical modification
  • Western blotting
  • Two-dimensional electrophoresis
  • ELISA
  • Ligand binding and fluorescence
  • Fluorescent DNA assays
  • Stopped-flow methods
  • Surface plasmon resonance

Readings

  • CHEM 477 course pack (explicitly required).
    Required
  • R. Eisenthal and M. J. Danson (eds.), Enzyme Assays: A Practical Approach (2002).
    Recommended / optional / reference
  • Alan Fersht, Structure and Mechanism in Protein Science: A Guide to Enzyme Catalysis and Protein Folding (1999).
    Recommended / optional / reference
  • Robert A. Copeland, Enzymes: A Practical Introduction to Structure, Mechanism, and Data Analysis (2000).
    Recommended / optional / reference
  • P. A. Frey and A. D. Hegeman, Enzymatic Reaction Mechanisms (2007).
    Recommended / optional / reference
  • A. J. Kirby and F. Hollfelder, From Enzyme Models to Model Enzymes (2009).
    Recommended / optional / reference
  • T. E. Creighton (ed.), Protein Function: A Practical Approach (1997).
    Recommended / optional / reference
  • A. Cooper, Biophysical Chemistry, 2nd ed. (2011).
    Recommended / optional / reference
  • A. M. Lesk, Introduction to Protein Science: Architecture, Function and Genomics, 2nd ed. (2010).
    Recommended / optional / reference
  • A. Kraj and J. Silberring (eds.), Proteomics: Introduction to Methods and Applications (2008).
    Recommended / optional / reference
  • Methods in Enzymology (reference series).
    Recommended / optional / reference
  • E. Buxbaum, Biophysical Chemistry of Proteins: An Introduction to Laboratory Methods.
    Recommended / optional / reference
  • I. Bahar, R. L. Jernigan and K. A. Dill, Protein Actions: Principles and Modeling.
    Recommended / optional / reference
  • N. S. Punekar, Enzymes: Catalysis, Kinetics and Mechanisms.
    Recommended / optional / reference
  • H. Morrison, Enzyme Active Sites and Their Reaction Mechanisms.
    Recommended / optional / reference
  • T. Skern, Exploring Protein Structure: Principles and Practice.
    Recommended / optional / reference
  • J. Lakowicz, Principles of Fluorescence Spectroscopy, 3rd ed.
    Recommended / optional / reference
  • Listed course manual: CHEM 477 Laboratory Manual.
    Listed reading
Sources