CHEM 477
Advanced Laboratory in Biochemistry
Fall 2021
Topics
- Protein structure and modelling
- Enzyme kinetics
- Chemical modification
- Western blotting
- Two-dimensional electrophoresis
- ELISA
- Ligand binding and fluorescence
- Fluorescent DNA assays
- Stopped-flow methods
- Surface plasmon resonance
Readings
- CHEM 477 course pack (explicitly required).
- R. Eisenthal and M. J. Danson (eds.), Enzyme Assays: A Practical Approach (2002).
- Alan Fersht, Structure and Mechanism in Protein Science: A Guide to Enzyme Catalysis and Protein Folding (1999).
- Robert A. Copeland, Enzymes: A Practical Introduction to Structure, Mechanism, and Data Analysis (2000).
- P. A. Frey and A. D. Hegeman, Enzymatic Reaction Mechanisms (2007).
- A. J. Kirby and F. Hollfelder, From Enzyme Models to Model Enzymes (2009).
- T. E. Creighton (ed.), Protein Function: A Practical Approach (1997).
- A. Cooper, Biophysical Chemistry, 2nd ed. (2011).
- A. M. Lesk, Introduction to Protein Science: Architecture, Function and Genomics, 2nd ed. (2010).
- A. Kraj and J. Silberring (eds.), Proteomics: Introduction to Methods and Applications (2008).
- Methods in Enzymology (reference series).
- E. Buxbaum, Biophysical Chemistry of Proteins: An Introduction to Laboratory Methods.
- I. Bahar, R. L. Jernigan and K. A. Dill, Protein Actions: Principles and Modeling.
- N. S. Punekar, Enzymes: Catalysis, Kinetics and Mechanisms.
- H. Morrison, Enzyme Active Sites and Their Reaction Mechanisms.
- T. Skern, Exploring Protein Structure: Principles and Practice.
- J. Lakowicz, Principles of Fluorescence Spectroscopy, 3rd ed.
- Listed course manual: CHEM 477 Laboratory Manual.
Sources
- Course catalogue ↗2026–2027
- Syllabus or course outline ↗Fall 2021
- Historical September–December 2021 course manual introduction. Suggested-reading books are reference/background resources; students receive additional experiment-specific readings separately. Dates above are printed in library call-number entries where available.