Cell Culture Basics: Microbial and Eukaryote Cells Laboratory

Graduate

This lab course covers foundational principles and hands-on techniques in microbial and eukaryotic cell culture, including growth kinetics, bioreactor operations, animal cell culture methods, gene transfer techniques, and tissue culture technologies such as flow cytometry and fluorescence microscopy. Cell growth cycles, cell cloning, microbial growth kinetics, antibiotic resistance Biology of cell culture: culture vessels, culture media, cell differentiation Bioreactor operations - batch, continuous, and fed-batch modes. Principles and technical skills fundamental to the culture of animal cells: sub-culturing, feeding, primary cultures and stable cell lines, cryopreservation, cell counting, cytotoxicity assays, cell viability assays, mycoplasma testing, Microsatellite Genotyping. Gene transfer techniques: chemical delivery, physical methods, and virus-mediated gene transfer. Tissue culture technologies: Flow cytometry, fluorescence microscopy. Offered Fall Term