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MBT 108

Genomics & Genome Editing Laboratory

Graduate

This lab course covers foundational genomics techniques alongside NGS-based bioinformatics analysis, single-cell and spatial transcriptomics, microbiome profiling, and genome editing methodologies such as CRISPR and gene therapy. DNA- and RNA extraction and QC, sequencing, libraries preparation, genome assembly, qPCR, RT-PCR, pairwise genome comparisons, gene expression profiles, genome-wide associations for disease discovery. Current NGS-based methodologies (including single cell) and the bioinformatics tools used to analyze sequence and genomic data in diverse areas of biology. Transcriptomic analysis (single cell), spatial transcriptomics, non-coding RNA. Microbiome: 16S profiling Genome editing methodologies (DNA repair, knock-out, knock-in, CRISPR, gene therapy). Offered Spring.

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