Introduction to Biomolecular Engineering (GE, BB, MC)

3 credits · Graduate

Techniques of molecular biology through linked lectures and laboratory exercises with emphasis on molecular tools to manipulate and analyze DNA and RNA for specific molecular bioengineering applications. Lectures cover the genetic code, replication, transcription, translation, cloning vectors for E. coli, enzymatic manipulation of DNA, gene cloning, synthetic gene design and assembly, DNA sequencing, polymerase chain reaction, site-directed mutagenesis, overexpression and purification of recombinant proteins. Laboratory exercises, linked to lectures, cover cloning, mutagenesis and recombinant protein expression and purification. Prerequisites: BIO 201L or BME 260L or graduate standing in BME.