Gene Silencing GenomeEditing

1 Credit · Level: Graduate, Undergraduate

Expression of each genes in eukaryotic cells is tightly regulated through various mechanism. Recently, researchers have developed several techniques to perturb specific gene expression either by manipulating at the RNA or DNA level. This course is designed to provide a comprehensive survey of different techniques currently used in biology to perturb specific gene expression. In this course, we will review organization of genes, gene expression, regulation of gene expression, techniques in gene silencing (siRNA, shRNA, miRNA, piRNA), genome editing (TALENS, ZFN, CRISPR, cDNA over expression) and applications of each techniques in medical sciences. n of each genes in eukaryotic cells is tightly regulated through various mechanism. Recently, researchers have developed several techniques to perturb specific gene expression either by manipulating at the RNA or DNA level. This course is designed to provide a comprehensive survey of different techniques currently used in biology to perturb specific gene expression. In this course, we will review organization of genes, gene expression, regulation of gene expression, techniques in gene silencing (siRNA, shRNA, miRNA, piRNA), genome editing (TALENS, ZFN, CRISPR, cDNA over expression) and applications of each techniques in medical sciences.