Biomed.Tiss.Eng. 3D Cell Cul

2 Credits · Level: Graduate

3-dimensional (3D) cell culture system is an important study model in drug discovery pipeline for its better mimicry of cellular microenvironment in vivo. This course will introduce students to essential cell culture concepts and techniques. In particular, it will focus on hands-on laboratory training of spheroid culture from tumor cells and organoid culture from primary cells. Different 3D culture techniques will be introduced including scaffold based and non-scaffold based. High throughput drug toxicity screening will be performed to examine effects in tumor proliferation, apoptosis, and invasion. Students will be exposed to the biomedical assays to screen for personalized medicine.