Experimental Meth in Biochem

3 Credits · Level: Graduate, Undergraduate

Students will work on a project that entails application of 5 to 8 modern techniques in biochemistry, including recombinant DNA methods, targeted gene expression in yeast or bacteria, subcellular fractionation/organelle purification, protein purification, gel electrophoresis and fluorescence spectroscopy. Combination of these techniques to perform an actual scientific project that would be typical for a modern biochemistry laboratory (e.g., expression, purification and structural analysis of a site-directed mutant protein). Prerequisite or concurrent: CHEM-419 or equivalent.