Genomics in the Lab

3 Credits · Level: Graduate, Undergraduate

The sequencing of the human genome was the beginning of the acceleration of the “big data” era in biology. We have seen technological advances and the availability of mobile sequencers that can generate DNA sequencing data in a matter of a few hours. The advent of these mobile sequencers has made it possible to generate sequencing data in a classroom. The objective of this course is to provide hands-on experience in using one such hand-held sequencer, the MinION to sequence a piece of DNA. and carry out Next-Generation Sequence analysis. Students will have a hands-on experience in the extraction, purification, loading of the DNA to the sequencer and the analysis of the Next Generation data collected from the sequencer. The course will be a combination of lectures and hands-on providing students in the “know-how” of DNA sequencing and analysis.