Introduction to Single-Molecule Biophysics

Harvard FAS

This course provides a practical introduction to single-molecule biophysics, a vibrant field that over the past several decades has transformed how we probe biological and soft-matter systems at the most fundamental level. We will build up the foundations of imaging science—including geometric and Fourier optics, light propagation and diffraction, resolution and transfer functions, noise and detectors, and the physics of fluorescence—before examining state-of-the-art single-molecule methods…