Microscopy

Harvard FAS

This course presents the fundamental concepts that underlie modern light microscopy in a rigorous but non-mathematical way for biological applications. The students will learn about the four major frameworks for light (ray optics, wave optics, electromagnetism, and quantum optics). The ways lenses work, the theory of resolution, and the optical design of the compound microscope will be described. The course will also describe the photo-physical principles that underlie fluorescence and…