Library Preparation in Next Generation Sequencing

4 Credits.

Next-generation sequencing (NGS) technology has revolutionized the field of genomics, enabling high-throughput analysis of DNA and RNA at unprecedented depths and speeds. However, the quality of NGS data is heavily dependent on the quality of the starting material and sample preparation methods used. This laboratory course aims to provide students with hands-on training in the most widely used NGS sample preparation techniques. The course will cover topics such as DNA and RNA extraction, library preparation, quality control, and quantification methods for NGS samples. The course will consist of both theoretical and practical sessions. In the theoretical sessions, students will learn about the underlying principles and theories behind each sample preparation method. In the practical sessions, students will be trained on laboratory techniques involved in NGS sample preparation, including the use of relevant equipment and software. Prerequisites: 410.656 Recombinant DNA Laboratory or approval of academic advisor.