Fundamentals of Fluorescence and Confocal Microscopy

1 Credit.

Teach fundamental concepts of fluorescence and confocal microscopy so that students can pose appropriate question for meaningful research results. Emphasis on computer-based tools, including ImageJ, Imaris and CellProfiler, for image preparation and analysis with sessions in computer lab almost every week, complementing lectures. Two additional lab days required for operating a generic fluorescence microscope and a confocal microscope, respectively, learning to avoid common errors that preclude quantitative image interpretation and evaluating signal-to-noise effects of confocal settings.