Morphological Techniques

3 cr · Graduate

The objective of this three credit hour course is to provide an overview of the basic techniques for morphological studies of cells and tissues. It is intended for graduate students interested in protein detection and protein localization in cells and tissues. In particular it is for students who want a deeper understanding of histological staining methods of tissues and immunodetection-based protein visualization in subcellular compartments. The course introduces to the principles antibody-based immunodetection methods in cells and tissues and provides a basic inroduction to brightfield and fluorescence microscopy. The course consists of lectures, microscopy demonstrations, and student led tutorials. The lectures will introduce to principle methods of histology staining and immunodetection methods. Tutorial sessions will provide a forum for student presentations and to discuss key considerations for the decisions for the use of antibodies, chemicals and fluorophores for cell- and tissue analyses. Microscopy sessions will provide a basic insight into considerations for image acquisition and basic image analysis.