Biochemical Research Techniques I

Undergraduate

examines the proteome and the genome. This course is designed to familiarize students with current methodology employed in the analyses of the complements of proteins and genes resident in eukaryotic cells. Emphasis will be placed on techniques that facilitate the simultaneous functional analyses of large numbers of proteins or genes. A variety of techniques, used in the study of expression and functional proteomics, will be described, including 2D PAGE, tagged proteins, fluorophores, mass spectrometry and protein microarrays. Techniques used in the study of gene expression and functional genomics will also be described, including the use of reporter gene constructs, analysis of protein-DNA interactions, expressions of cloned genes and several experimental approaches used to define the eukaryotic transcriptome.