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10.442

Biochemical Engineering and Biomanufacturing Principles

3-0-6 units · Undergraduate

Explores the interactions of chemical engineering, biochemical engineering, and microbiology with particular emphasis on applications to bioprocess development. Examines mathematical representations of microbial systems, especially with regard to the kinetics of growth, death, and metabolism. Discusses the fundamentals of bioreactor design and operation, including continuous fermentation, mass transfer, and agitation. Examples encompass both enzyme and whole cell systems. Presents concepts in process development for microbial and animal cell cultures, with considerations towards production of biological products ranging from chiral specialty chemicals/pharmaceuticals to therapeutic proteins. Concludes with a discussion of aspects of cellular engineering and the role of molecular biology in addressing process development problems.

Spring 2005 · Biochemical Engineering

Topics

  • Course Overview, Enzyme Classification
  • Enzyme Kinetics (Single-substrate Reactions), Measurement
  • Enzyme Inhibition
  • Enzyme Inhibition, pH and Temp Effects
  • Non-ideal Enzyme Kinetics
  • Immobilized Enzyme Kinetics - External
  • Immobilized Enzyme Kinetics - Internal
  • Applications of Enzyme Catalysis (Biocatalysis)
  • Microbial Growth
  • Microbial Growth - Batch Cultivation Profiles
  • Stoichiometry of Growth, Product Formation
  • Stoichiometry of Growth, Product Formation (cont.)
  • Models of Microbial Growth
  • Models of Microbial Growth (cont.)
  • Continuous Culture
  • Continuous Culture (cont.)
  • Bioreactor Design
  • Bioreactor Design (cont.)
  • Mass Transfer
  • Mass Transfer (cont.)
  • Heat Transfer, Media and Air Sterilization
  • Scale-up
  • Scale-down
  • Animal Cell Culture
  • Animal Cell Culture (cont.)
  • Mixed Cultures
  • Mixed Cultures (cont.)
  • Medium Development
  • Medium Development (cont.)
  • Cellular Engineering - An Engineer’s View Towards Recombinant Organisms
  • Cellular Engineering/Molecular Biology for Process Engineering
  • Cellular Engineering/Molecular Biology for Process Engineering (cont.)
  • Enzyme Engineering for Process Improvement
  • Enzyme Engineering for Process Improvement (cont.)

Readings

  • [S&K] Shuler, Michael L., and Fikret Kargi. Bioprocess Engineering: Basic Concepts . 2nd ed. Upper Saddle River, NJ: Prentice Hall PTR, 2001. ISBN: 9780130819086.
    Required
  • Blanch, Harvey W., and D. S. Clark, eds. Biochemical Engineering . New York, NY: Marcel Dekker Incorporated, 1997. ISBN: 9780824700997.
    Listed reading
  • Bailey, James E., and David F. Ollis. Biochemical Engineering Fundamentals. New York, NY: McGraw-Hill Education, 1986. ISBN: 9780070666016.
    Listed reading
  • Rozzell, J. D. “Commercial scale biocatalysis: myths and realities.” Bioorg Med Chem 7, no. 10 (October 1999): 2253-61.
    Listed reading
  • SES #: 1 · TOPICS: Course Overview, Enzyme Classification · READINGS: S&K. Chapter 1.
    Reading assignment
  • SES #: 2 · TOPICS: Enzyme Kinetics (Single-substrate Reactions), Measurement · READINGS: S&K. Chapter 3.1-3.3.3.
    Reading assignment
  • SES #: 3 · TOPICS: Enzyme Inhibition · READINGS: S&K. Chapter 3.3.4.
    Reading assignment
  • SES #: 4 · TOPICS: Enzyme Inhibition, pH and Temp Effects · READINGS: S&K. Chapter 3.3.5.
    Reading assignment
  • SES #: 5 · TOPICS: Non-ideal Enzyme Kinetics · READINGS: Class notes
    Reading assignment
  • SES #: 6 · TOPICS: Immobilized Enzyme Kinetics - External · READINGS: S&K. Chapter 3.4.
    Reading assignment
  • SES #: 7 · TOPICS: Immobilized Enzyme Kinetics - Internal · READINGS: S&K. Chapter 3.4.
    Reading assignment
  • SES #: 8 · TOPICS: Applications of Enzyme Catalysis (Biocatalysis) · READINGS: S&K. Chapter 3.5, 3.6.
    Reading assignment
  • SES #: 9 · TOPICS: Microbial Growth · READINGS: S&K. Chapter 6.1-6.2.
    Reading assignment
  • SES #: 10 · TOPICS: Microbial Growth - Batch Cultivation Profiles · READINGS: S&K. Chapter 6.1-6.2.
    Reading assignment
  • SES #: 11 · TOPICS: Stoichiometry of Growth, Product Formation · READINGS: S&K. Chapter 7.
    Reading assignment
  • SES #: 12 · TOPICS: Stoichiometry of Growth, Product Formation (cont.) · READINGS: S&K. Chapter 7.
    Reading assignment
  • SES #: 13 · TOPICS: Models of Microbial Growth · READINGS: S&K. Chapter 6.3.
    Reading assignment
  • SES #: 14 · TOPICS: Models of Microbial Growth (cont.) · READINGS: S&K. Chapter 6.3.
    Reading assignment
  • SES #: 15 · TOPICS: Continuous Culture · READINGS: S&K. Chapter 6.4.
    Reading assignment
  • SES #: 16 · TOPICS: Continuous Culture (cont.) · READINGS: S&K. Chapter 6.4.
    Reading assignment
  • SES #: 17 · TOPICS: Bioreactor Design · READINGS: S&K. Chapter 9.1-9.3.
    Reading assignment
  • SES #: 18 · TOPICS: Bioreactor Design (cont.) · READINGS: S&K. Chapter 9.1-9.3.
    Reading assignment
  • SES #: 20 · TOPICS: Mass Transfer · READINGS: S&K. Chapter 6.2.3-6.2.4, 10.2.3.
    Reading assignment
  • SES #: 21 · TOPICS: Mass Transfer (cont.) · READINGS: S&K. Chapter 6.2.3-6.2.4, 10.2.3.
    Reading assignment
  • SES #: 22 · TOPICS: Heat Transfer, Media and Air Sterilization · READINGS: S&K. Chapter 10.4.
    Reading assignment
  • SES #: 23 · TOPICS: Scale-up · READINGS: S&K. Chapter 10.2.4.
    Reading assignment
  • SES #: 24 · TOPICS: Scale-down · READINGS: S&K. Chapter 10.2.5.
    Reading assignment
  • SES #: 25 · TOPICS: Animal Cell Culture · READINGS: S&K. Chapter 12.
    Reading assignment
  • SES #: 26 · TOPICS: Animal Cell Culture (cont.) · READINGS: S&K. Chapter 12.
    Reading assignment
  • SES #: 27 · TOPICS: Mixed Cultures · READINGS: S&K. Chapter 16.
    Reading assignment
  • SES #: 28 · TOPICS: Mixed Cultures (cont.) · READINGS: S&K. Chapter 16.
    Reading assignment
  • SES #: 29 · TOPICS: Medium Development · READINGS: Handouts
    Reading assignment
  • SES #: 30 · TOPICS: Medium Development (cont.) · READINGS: Handouts
    Reading assignment
  • SES #: 31 · TOPICS: Cellular Engineering - An Engineer’s View Towards Recombinant Organisms · READINGS: S&K. Chapter 14.
    Reading assignment
  • SES #: 32 · TOPICS: Cellular Engineering/Molecular Biology for Process Engineering · READINGS: S&K. Chapter 14.
    Reading assignment
  • SES #: 33 · TOPICS: Cellular Engineering/Molecular Biology for Process Engineering (cont.) · READINGS: S&K. Chapter 14.
    Reading assignment
  • SES #: 34 · TOPICS: Enzyme Engineering for Process Improvement · READINGS: Handouts
    Reading assignment
  • SES #: 35 · TOPICS: Enzyme Engineering for Process Improvement (cont.) · READINGS: Handouts
    Reading assignment
  • Shuler, Michael L., and Fikret Kargi. Bioprocess Engineering: Basic Concepts. 2nd ed. Upper Saddle River, NJ: Prentice Hall PTR, 2001. ISBN: 9780130819086.
    Required
  • Blanch, Harvey W., and D. S. Clark, eds. Biochemical Engineering . New York, NY: Marcel Dekker Incorporated, 1997. ISBN: 9780824700997.
    Required
  • Bailey, James E., and David F. Ollis. Biochemical Engineering Fundamentals . New York, NY: McGraw-Hill Education, 1986. ISBN: 9780070666016.
    Required

Prerequisites & course information

Subject meets with 10.542 Prereq: ( Biology (GIR) , 5.07[J] , and 10.37 ) or permission of instructor U (Spring) Not offered regularly; consult department 3-0-6 units

Sources