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10.445

Separation Processes for Biochemical Products

Undergraduate · Historical course

Summer 2005

Topics

  • Strategies for Biochemical Process Synthesis
  • Biochemical Processing Overview
  • Cell Disruption
  • Solid-Liquid Separation: Centrifugation
  • Chromatography
  • Protein Refolding
  • Solid-Liquid Separation: Filtration
  • Membrane and Filtration Applications
  • Rotary Vacuum Filtration
  • Process Synthesis, Analysis and Simulation
  • Process Simulation
  • Case Study in Innovation
  • Process Validation

Readings

  • Shutte, H., and M. R. Kula. “Cell Disruption and Isolation of Non-Secreted Products.” In Biotechnology: A Multi-Volume Comprehensive Treatise . Vol. 3, Bioprocessing . Edited by G. Stephanopoulos. New York, NY: VCH Publishers, Inc., 1993, pp. 505-526. ISBN: 9783527283132.
    Listed reading
  • Datar, R. V., and C. G. Rosen. “Cell and Cell Debris Removal: Centrifugation and Crossflow Filtration.” In Biotechnology: A Multi-Volume Comprehensive Treatise . Vol. 3, Bioprocessing . Edited by G. Stephanopoulos. New York, NY: VCH Publishers, Inc., 1996, pp. 469-486. ISBN: 9783527283132.
    Listed reading
  • Palmer, D. E., and R. V. Dove. “Ion Exchange Recovery of Proteins.” In Comprehensive Biotechnology . Vol. 2, The Principles of Biotechnology: Engineering Considerations . Edited by C. L. Cooney, and A. E. Humphrey. Elmsford, NY: Pergamon Press Ltd., 1985, pp. 481-488. ISBN: 008032510.
    Listed reading
  • Yarmush, M. L., K. Antonsen, and D. M. Yarmush. “Molecular Sieve Chromatography.” In Comprehensive Biotechnology . Vol. 2, The Principles of Biotechnology: Engineering Considerations . Edited by C. L. Cooney, and A. E. Humphrey. Elmsford, NY: Pergamon Press Ltd., 1985, pp. 489-505. ISBN: 008032510.
    Listed reading
  • Skoog, D. A., D. M. West, and F. J. Holler. “An Introduction to Chromatographic Methods.” In Fundamentals of Analytical Chemistry . New York, NY: Saunders College Publishing, 1992, pp. 665-693. ISBN: 9780030749223.
    Listed reading
  • Cleland, J. L., and D. I. C. Wang. “ In vitro Protein Refolding.” In Biotechnology: A Multi-Volume Comprehensive Treatise . Vol. 3, Bioprocessing . Edited by G. Stephanopoulos. New York, NY: VCH Publishers, Inc., 1996, pp. 527-555. ISBN: 9783527283132.
    Listed reading
  • Datar, R. V., T. Cartwright, and C. G. Rosen. “Process Economics of Animal Cell and Bacterial Fermentations - A Case Study Analysis of Tissue Plasminogen Activator.” Bio/Technology 11, no. 3 (1993) 349-357.
    Listed reading
  • Ladisch, M. R. “Filtration.” In Bioseparations Engineering: Principles, Practice, and Economics . New York, NY: John Wiley and Sons, 2001, pp. 36-47. ISBN: 9780471244769.
    Listed reading
  • Datar, R. V., and C. G. Rosen. “Cell and Cell Debris Removal: Centrifugation and Crossflow Filtration.” In Biotechnology: A Multi-Volume Comprehensive Treatise . Vol. 3, Bioprocessing . Edited by G. Stephanopoulos. New York, NY: VCH Publishers, Inc., 1996, pp. 487-503. ISBN: 9783527283132.
    Listed reading
  • Tutunjian, R. S. “Ultrafiltration Processes in Biotechnology.” In Comprehensive Biotechnology . Vol. 2, The Principles of Biotechnology: Engineering Considerations . Edited by C. L. Cooney, and A. E. Humphrey. Elmsford, NY: Pergamon Press Ltd., 1985, pp. 411-437. ISBN: 008032510.
    Listed reading
  • Shuler, M. L., and F. Kargi. “Separation of Soluble Products.” In Bioprocess Engineeing . Englewood Cliffs, NJ: Prentice Hall PTR, 1991, pp. 325-331. ISBN: 9780134782157.
    Listed reading
  • Kelly, B. D., and T. A. Hatton. “Protein Purification by Liquid-Liquid Extraction.” In Biotechnology: A Multi-Volume Comprehensive Treatise . Vol. 3, Bioprocessing . Edited by G. Stephanopoulos. New York, NY: VCH Publishers, Inc., 1996, pp. 593-616. ISBN: 9783527283132.
    Listed reading
  • Petrides, D., C. L. Cooney, and L. B. Evans. “An Introduction to Biochemical Process Design.” In Chemical Engineering Problems in Biotechnology . Edited by M. L. Shuler. New York, NY: American Institute of Chemical Engineers, 1989, pp. 351-391. ISBN: 9780816904693.
    Listed reading
  • Ernst, S., et al. “Process Simulation for Recombinant Protein Production: Cost Estimation and Sensitivity Analysis for Heparinase I Expressed in Escherichia Coli .” Biotechnology and Bioengineering 53, no. 6 (1997): 575-582.
    Listed reading
  • _Insulin Case Study _ Ladisch, M. R., and K. L. Kohlmann. “Recombinant Human Insulin.” Biotechnology Progress 8, no. 6 (1992): 469-478.
    Listed reading
  • Petrides, D., E. Sapidou, and J. Calandranis. “Computer-Aided Process Analysis and Economic-Evaluation for Biosynthetic Human Insulin Production - A Case Study.” Biotechnology and Bioengineering 48, no. 5 (1995): 529-541.
    Listed reading
  • Shutte, H., and M. R. Kula. “Cell Disruption and Isolation of Non-Secreted Products.” In Biotechnology: A Multi-Volume Comprehensive Treatise . Vol. 3, Bioprocessing . Edited by G. Stephanopoulos. New York, NY: VCH Publishers, Inc., 1996, pp. 505-526. ISBN: 9783527283132.
    Required
  • Datar, R. V., and C. G. Rosen. “Cell Debris Removal: Centrifugation and Crossflow Filtration.” In Biotechnology: A Multi-Volume Comprehensive Treatise . Vol. 3, Bioprocessing . Edited by G. Stephanopoulos. New York, NY: VCH Publishers, Inc., 1996, pp. 469-503. ISBN: 9783527283132.
    Required
  • Palmer, D. E., and R. V. Dove. “Ion Exchange Recovery of Proteins.” In Comprehensive Biotechnology . Vol. 2, The Principles of Biotechnology: Engineering Considerations . Edited by C. L. Cooney, and A. E. Humphrey. Elmsford, NY: Pergamon Press Ltd., 1985, pp. 481-488. ISBN: 008032510.
    Required
  • Yarmush, M. L., K. Antonsen, and D. M. Yarmush. “Molecular Sieve Chromatography.” In Comprehensive Biotechnology . Vol. 2, The Principles of Biotechnology: Engineering Considerations . Edited by C. L. Cooney, and A. E. Humphrey. Elmsford, NY: Pergamon Press Ltd., 1985, pp. 489-505. ISBN: 008032510.
    Required
  • Skoog, D. A., D. M. West, and F. J. Holler. “An Introduction to Chromatographic Methods.” In Fundamentals of Analytical Chemistry . New York, NY: Saunders College Publishing, 1992, pp. 665-693. ISBN: 9780030749223.
    Required
  • Cleland, J. L., and D. I. C. Wang. “In vitro Protein Refolding.” In Biotechnology: A Multi-Volume Comprehensive Treatise . Vol. 3, Bioprocessing . Edited by G. Stephanopoulos. New York, NY: VCH Publishers, Inc., 1996, pp. 527-555. ISBN: 9783527283132.
    Required
  • Datar, R. V., T. Cartwright, and C. G. Rosen. “Process Economics of Animal Cell and Bacterial Fermentations - A Case Study Analysis of Tissue Plasminogen Activator.” Bio/Technology 11, no. 3 (1993) 349-357.
    Required
  • Ladisch, M. R. “Filtration.” In Bioseparations Engineering: Principles, Practice, and Economics . New York, NY: John Wiley and Sons, 2001, pp. 36-48. ISBN: 9780471244769.
    Required
  • Tutunjian, R. S. “Ultrafiltration Processes in Biotechnology.” In Comprehensive Biotechnology . Vol. 2, The Principles of Biotechnology: Engineering Considerations . Edited by C. L. Cooney, and A. E. Humphrey. Elmsford, NY: Pergamon Press Ltd., 1985, pp. 411-437. ISBN: 008032510.
    Required
  • Shuler, M. L., and F. Kargi. “Separation of Soluble Products.” In Bioprocess Engineeing . Englewood Cliffs, NJ: Prentice Hall PTR, 1991, pp. 325-331. ISBN: 9780134782157.
    Required
  • Kelly, B. D., and T. A. Hatton. “Protein Purification by Liquid-Liquid Extraction.” In Biotechnology: A Multi-Volume Comprehensive Treatise . Vol. 3, Bioprocessing . Edited by G. Stephanopoulos. New York, NY: VCH Publishers, Inc., 1993, pp. 593-616. ISBN: 9783527283132.
    Required
  • Petrides, D., C. L. Cooney, and L. B. Evans. “An Introduction to Biochemical Process Design.” In Chemical Engineering Problems in Biotechnology . Edited by M. L. Shuler. New York, NY: American Institute of Chemical Engineers, 1989, pp. 351-391. ISBN:9780816904693.
    Required
  • Ernst, S., et al. “Process Simulation for Recombinant Protein Production: Cost Estimation and Sensitivity Analysis for Heparinase I Expressed in Escherichia coli.” Biotechnology and Bioengineering 53, no. 6 (1997): 575-582.
    Required
  • Ladisch, M. R., and K. L. Kohlmann. “Recombinant Human Insulin.” Biotechnology Progress 8, no. 6 (1992): 469-478.
    Required
  • Petrides, D., E. Sapidou, and J. Calandranis. “Computer-Aided Process Analysis and Economic-Evaluation for Biosynthetic Human Insulin Production - A Case Study.” Biotechnology and Bioengineering 48, no. 5 (1995): 529-541.
    Required
Sources