everything.study
10.545

Fundamentals of Metabolic and Biochemical Engineering: Applications to Biomanufacturing

3-0-9 units · Graduate

Examines the fundamentals of cell and metabolic engineering for biocatalyst design and optimization, as well as biochemical engineering principles for bioreactor design and operation, and downstream processing. Presents applications of microbial processes for production of commodity and specialty chemicals and biofuels in addition to mammalian cell cultures for production of biopharmaceuticals. Students taking graduate version complete additional assignments.

Summer 2005 · Separation Processes for Biochemical Products

Topics

  • Strategies for Biochemical Process Synthesis
  • Biochemical Processing Overview
  • Cell Disruption
  • Solid-Liquid Separation: Centrifugation
  • Chromatography
  • Protein Refolding
  • Solid-Liquid Separation: Filtration
  • Membrane and Filtration Applications
  • Rotary Vacuum Filtration
  • Process Synthesis, Analysis and Simulation
  • Process Simulation
  • Case Study in Innovation
  • Process Validation

Readings

  • Shutte, H., and M. R. Kula. “Cell Disruption and Isolation of Non-Secreted Products.” In Biotechnology: A Multi-Volume Comprehensive Treatise . Vol. 3, Bioprocessing . Edited by G. Stephanopoulos. New York, NY: VCH Publishers, Inc., 1993, pp. 505-526. ISBN: 9783527283132.
    Listed reading
  • Datar, R. V., and C. G. Rosen. “Cell and Cell Debris Removal: Centrifugation and Crossflow Filtration.” In Biotechnology: A Multi-Volume Comprehensive Treatise . Vol. 3, Bioprocessing . Edited by G. Stephanopoulos. New York, NY: VCH Publishers, Inc., 1996, pp. 469-486. ISBN: 9783527283132.
    Listed reading
  • Palmer, D. E., and R. V. Dove. “Ion Exchange Recovery of Proteins.” In Comprehensive Biotechnology . Vol. 2, The Principles of Biotechnology: Engineering Considerations . Edited by C. L. Cooney, and A. E. Humphrey. Elmsford, NY: Pergamon Press Ltd., 1985, pp. 481-488. ISBN: 008032510.
    Listed reading
  • Yarmush, M. L., K. Antonsen, and D. M. Yarmush. “Molecular Sieve Chromatography.” In Comprehensive Biotechnology . Vol. 2, The Principles of Biotechnology: Engineering Considerations . Edited by C. L. Cooney, and A. E. Humphrey. Elmsford, NY: Pergamon Press Ltd., 1985, pp. 489-505. ISBN: 008032510.
    Listed reading
  • Skoog, D. A., D. M. West, and F. J. Holler. “An Introduction to Chromatographic Methods.” In Fundamentals of Analytical Chemistry . New York, NY: Saunders College Publishing, 1992, pp. 665-693. ISBN: 9780030749223.
    Listed reading
  • Cleland, J. L., and D. I. C. Wang. “ In vitro Protein Refolding.” In Biotechnology: A Multi-Volume Comprehensive Treatise . Vol. 3, Bioprocessing . Edited by G. Stephanopoulos. New York, NY: VCH Publishers, Inc., 1996, pp. 527-555. ISBN: 9783527283132.
    Listed reading
  • Datar, R. V., T. Cartwright, and C. G. Rosen. “Process Economics of Animal Cell and Bacterial Fermentations - A Case Study Analysis of Tissue Plasminogen Activator.” Bio/Technology 11, no. 3 (1993) 349-357.
    Listed reading
  • Ladisch, M. R. “Filtration.” In Bioseparations Engineering: Principles, Practice, and Economics . New York, NY: John Wiley and Sons, 2001, pp. 36-47. ISBN: 9780471244769.
    Listed reading
  • Datar, R. V., and C. G. Rosen. “Cell and Cell Debris Removal: Centrifugation and Crossflow Filtration.” In Biotechnology: A Multi-Volume Comprehensive Treatise . Vol. 3, Bioprocessing . Edited by G. Stephanopoulos. New York, NY: VCH Publishers, Inc., 1996, pp. 487-503. ISBN: 9783527283132.
    Listed reading
  • Tutunjian, R. S. “Ultrafiltration Processes in Biotechnology.” In Comprehensive Biotechnology . Vol. 2, The Principles of Biotechnology: Engineering Considerations . Edited by C. L. Cooney, and A. E. Humphrey. Elmsford, NY: Pergamon Press Ltd., 1985, pp. 411-437. ISBN: 008032510.
    Listed reading
  • Shuler, M. L., and F. Kargi. “Separation of Soluble Products.” In Bioprocess Engineeing . Englewood Cliffs, NJ: Prentice Hall PTR, 1991, pp. 325-331. ISBN: 9780134782157.
    Listed reading
  • Kelly, B. D., and T. A. Hatton. “Protein Purification by Liquid-Liquid Extraction.” In Biotechnology: A Multi-Volume Comprehensive Treatise . Vol. 3, Bioprocessing . Edited by G. Stephanopoulos. New York, NY: VCH Publishers, Inc., 1996, pp. 593-616. ISBN: 9783527283132.
    Listed reading
  • Petrides, D., C. L. Cooney, and L. B. Evans. “An Introduction to Biochemical Process Design.” In Chemical Engineering Problems in Biotechnology . Edited by M. L. Shuler. New York, NY: American Institute of Chemical Engineers, 1989, pp. 351-391. ISBN: 9780816904693.
    Listed reading
  • Ernst, S., et al. “Process Simulation for Recombinant Protein Production: Cost Estimation and Sensitivity Analysis for Heparinase I Expressed in Escherichia Coli .” Biotechnology and Bioengineering 53, no. 6 (1997): 575-582.
    Listed reading
  • _Insulin Case Study _ Ladisch, M. R., and K. L. Kohlmann. “Recombinant Human Insulin.” Biotechnology Progress 8, no. 6 (1992): 469-478.
    Listed reading
  • Petrides, D., E. Sapidou, and J. Calandranis. “Computer-Aided Process Analysis and Economic-Evaluation for Biosynthetic Human Insulin Production - A Case Study.” Biotechnology and Bioengineering 48, no. 5 (1995): 529-541.
    Listed reading
  • Shutte, H., and M. R. Kula. “Cell Disruption and Isolation of Non-Secreted Products.” In Biotechnology: A Multi-Volume Comprehensive Treatise . Vol. 3, Bioprocessing . Edited by G. Stephanopoulos. New York, NY: VCH Publishers, Inc., 1996, pp. 505-526. ISBN: 9783527283132.
    Required
  • Datar, R. V., and C. G. Rosen. “Cell Debris Removal: Centrifugation and Crossflow Filtration.” In Biotechnology: A Multi-Volume Comprehensive Treatise . Vol. 3, Bioprocessing . Edited by G. Stephanopoulos. New York, NY: VCH Publishers, Inc., 1996, pp. 469-503. ISBN: 9783527283132.
    Required
  • Palmer, D. E., and R. V. Dove. “Ion Exchange Recovery of Proteins.” In Comprehensive Biotechnology . Vol. 2, The Principles of Biotechnology: Engineering Considerations . Edited by C. L. Cooney, and A. E. Humphrey. Elmsford, NY: Pergamon Press Ltd., 1985, pp. 481-488. ISBN: 008032510.
    Required
  • Yarmush, M. L., K. Antonsen, and D. M. Yarmush. “Molecular Sieve Chromatography.” In Comprehensive Biotechnology . Vol. 2, The Principles of Biotechnology: Engineering Considerations . Edited by C. L. Cooney, and A. E. Humphrey. Elmsford, NY: Pergamon Press Ltd., 1985, pp. 489-505. ISBN: 008032510.
    Required
  • Skoog, D. A., D. M. West, and F. J. Holler. “An Introduction to Chromatographic Methods.” In Fundamentals of Analytical Chemistry . New York, NY: Saunders College Publishing, 1992, pp. 665-693. ISBN: 9780030749223.
    Required
  • Cleland, J. L., and D. I. C. Wang. “In vitro Protein Refolding.” In Biotechnology: A Multi-Volume Comprehensive Treatise . Vol. 3, Bioprocessing . Edited by G. Stephanopoulos. New York, NY: VCH Publishers, Inc., 1996, pp. 527-555. ISBN: 9783527283132.
    Required
  • Datar, R. V., T. Cartwright, and C. G. Rosen. “Process Economics of Animal Cell and Bacterial Fermentations - A Case Study Analysis of Tissue Plasminogen Activator.” Bio/Technology 11, no. 3 (1993) 349-357.
    Required
  • Ladisch, M. R. “Filtration.” In Bioseparations Engineering: Principles, Practice, and Economics . New York, NY: John Wiley and Sons, 2001, pp. 36-48. ISBN: 9780471244769.
    Required
  • Tutunjian, R. S. “Ultrafiltration Processes in Biotechnology.” In Comprehensive Biotechnology . Vol. 2, The Principles of Biotechnology: Engineering Considerations . Edited by C. L. Cooney, and A. E. Humphrey. Elmsford, NY: Pergamon Press Ltd., 1985, pp. 411-437. ISBN: 008032510.
    Required
  • Shuler, M. L., and F. Kargi. “Separation of Soluble Products.” In Bioprocess Engineeing . Englewood Cliffs, NJ: Prentice Hall PTR, 1991, pp. 325-331. ISBN: 9780134782157.
    Required
  • Kelly, B. D., and T. A. Hatton. “Protein Purification by Liquid-Liquid Extraction.” In Biotechnology: A Multi-Volume Comprehensive Treatise . Vol. 3, Bioprocessing . Edited by G. Stephanopoulos. New York, NY: VCH Publishers, Inc., 1993, pp. 593-616. ISBN: 9783527283132.
    Required
  • Petrides, D., C. L. Cooney, and L. B. Evans. “An Introduction to Biochemical Process Design.” In Chemical Engineering Problems in Biotechnology . Edited by M. L. Shuler. New York, NY: American Institute of Chemical Engineers, 1989, pp. 351-391. ISBN:9780816904693.
    Required
  • Ernst, S., et al. “Process Simulation for Recombinant Protein Production: Cost Estimation and Sensitivity Analysis for Heparinase I Expressed in Escherichia coli.” Biotechnology and Bioengineering 53, no. 6 (1997): 575-582.
    Required
  • Ladisch, M. R., and K. L. Kohlmann. “Recombinant Human Insulin.” Biotechnology Progress 8, no. 6 (1992): 469-478.
    Required
  • Petrides, D., E. Sapidou, and J. Calandranis. “Computer-Aided Process Analysis and Economic-Evaluation for Biosynthetic Human Insulin Production - A Case Study.” Biotechnology and Bioengineering 48, no. 5 (1995): 529-541.
    Required

Prerequisites & course information

Subject meets with 10.345 Prereq: 5.07[J] , 7.05 , or permission of instructor G (Spring) Not offered regularly; consult department 3-0-9 units

Sources