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20.450

Applied Microbiology

4-0-8 units · Graduate

Compares the complex molecular and cellular interactions in health and disease between commensal microbial communities, pathogens and the human or animal host. Special focus is given to current research on microbe/host interactions, infection of significant importance to public health, and chronic infectious disease. Classwork will include lecture, but emphasize critical evaluation and class discussion of recent scientific papers, and the development of new research agendas in the fields presented.

Spring 2005 · Molecular and Cellular Pathophysiology (BE.450)

Topics

  • Introduction to 20.450 and HCC
  • Cellular Pathology
  • Inflammation
  • Liver Anatomy and Histology
  • Immunity
  • Neoplasia
  • Neoplasia (cont.)
  • Infectious Diseases
  • Liver and Biliary
  • Hepatocarcinogenesis
  • Animal Models
  • Special Topic

Readings

  • Kumar, Vinay, Abul K. Abbas, and Nelson Fausto, eds. Robbins and Cotran Pathologic Basis of Disease . 7th ed. Philadelphia, PA: Elsevier Saunders, 2004. ISBN: 9780721601878.
    Required
  • Sherlock, G. “Analysis of large-scale gene expression data.” Current Opinion in Immunology 12, no. 2 (April 2000): 201-5. Review.
    Required
  • LEC #: 2-3 · TOPICS: Cellular Pathology · READINGS: “Cellular Adaptations, Cell Injury, and Cell Death.” Chapter 1 in Robbins and Cotran. pp. 3-46.
    Reading assignment
  • “Acute and Chronic Inflammation. ” Chapter 2 in Robbins and Cotran. pp. 47-86.
    Required
  • Coussens, L. M., and Z. Werb. “Inflammation and cancer.” Nature 420, no. 6917 (December 19-26, 2002): 860-7. Review.
    Required
  • Muller, A., et al. “Involvement of chemokine receptors in breast cancer metastasis.” Nature 410, no. 6824 (March 1, 2001): 50-6.
    Required
  • Liotta, L. A. “An attractive force in metastasis.” Nature 410, no. 6824 (March 1, 2001): 24-5.
    Required
  • “Liver and Biliary Tract.” Chapter 18 in Robbins and Cotran . pp. 878-879.
    Required
  • “Tissue Renewal and Repair: Regeneration, Healing, and Fibrosis.” Chapter 3 in Robbins and Cotran . pp. 87-118.
    Required
  • “Diseases of Immunity.” Chapter 6 in Robbins and Cotran . pp. 193-240.
    Required
  • Prasad, S. J., et al. “Dendritic cells loaded with stressed tumor cells elicit long-lasting protective tumor immunity in mice depleted of CD4+CD25+ regulatory T cells.” Journal of Immunology 174, no. 1 (January 1, 2005): 90-8.
    Required
  • Rehermann, B., and M. Nascimbeni. “Immunology of hepatitis B virus and hepatitis C virus infection.” Nature Review Immunology 5, no. 3 (March 2005): 215-29.
    Required
  • Crispe, I. N. “Hepatic T cells and liver tolerance.” Nature Review Immunology 3, no. 1 (January 2003): 51-62.
    Required
  • “Neoplasia. ” Chapter 7 in Robbins and Cotran . pp. 269-339.
    Required
  • Li, et al. “Accurate qualitative and quantitative proteomic analysis of clinical hepatocellular carcinoma using laser capture microdissection coupled with isotope-coded affinity tag and two-dimensional liquid chromatography mass spectrometry.” Molecular and Cellular Proteomics 3, no. 4 (April 2004): 399-409.
    Required
  • Ding, S. J., et al. “From proteomic analysis to clinical significance: overexpression of cytokeratin 19 correlates with hepatocellular carcinoma metastasis.” Molecular and Cellular Proteomics 3, no. 1 (January 2004): 73-81.
    Required
  • Staib, F., S. P. Hussain, L. J. Hofseth, X. W. Wang, and C. C. Harris. “TP53 and liver carcinogenesis.” Human Mutation 21, no. 3 (March 2003): 201-16. Review.
    Required
  • LEC #: 11 · TOPICS: Neoplasia (cont.) · READINGS: See readings for above Neoplasia lecture
    Reading assignment
  • LEC #: 12-13 · TOPICS: Infectious Diseases · READINGS: “Infectious Diseases.” Chapter 8 in Robbins and Cotran . pp. 343-411.
    Reading assignment
  • “Liver and Biliary Tract.” Chapter 18 in Robbins and Cotran . pp. 877-936.
    Required
  • Carey, M. C., R. S. Kwon, K. J. Maurer, and J. G. Fox. “State of the art - gallstone research in the post-genomic era.” Falk Symposium 139 (2004): 207-25.
    Required
  • Rogers, A. B., and J. G. Fox. “Inflammation and Cancer. I. Rodent models of infectious gastrointestinal and liver cancer.” American Journal of Physiology - Gastrointestinal and Liver Physiology 286, no. 3 (March 2004): G361-6. Review.
    Required
  • Iizuka, N., et al. “Self-organizing-map-based molecular signature representing the development of hepatocellular carcinoma.” FEBS Letters 579, no. 5 (February 14, 2005): 1089-100.
    Required
  • Wielockx, B., K. Lannoy, S. D. Shapiro, T. Itoh, S. Itohara, J. Vandekerckhove, and C. Libert. “Inhibition of matrix metalloproteinases blocks lethal hepatitis and apoptosis induced by tumor necrosis factor and allows safe antitumor therapy.” Nature Medicine 7, no. 11 (November 2001): 1202-8.
    Required
  • Benyon, R. C., and M. J. Arthur. “Extracellular matrix degradation and the role of hepatic stellate cells.” Seminars in Liver Disease 21, no. 3 (August 2001): 373-84.
    Required
  • Kensler, T. W., P. A. Egner, J. B. Wang, Y. R. Zhu, B. C. Zhang, P. X. Lu, J. G. Chen, G. S. Qian, S. Y. Kuang, P. E. Jackson, S. J. Gange, L. P. Jacobson, A. Munoz, and J. D. Groopman. “Chemoprevention of hepatocellular carcinoma in aflatoxin endemic areas.” Gastroenterology 127, no. 5 Suppl. 1 (November 2004): S310-8.
    Required
  • Readings are in the form of primary scientific papers, reviews, and selected chapters from the following text:
    Listed reading
  • Kumar, Vinay, Abul K. Abbas, and Nelson Fausto, eds. Robbins and Cotran Pathologic Basis of Disease . 7th ed. Philadelphia, PA: Elsevier Saunders, 2004. ISBN: 9780721601878.
    Listed reading

Prerequisites & course information

Prereq: ( 20.420[J] and 20.440 ) or permission of instructor G (Fall) 4-0-8 units

Sources