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7.72

Stem Cells, Regeneration, and Development

4-0-8 units · Graduate

Topics include diverse stem cells, such as muscle, intestine, skin, hair and hematopoietic stem cells, as well as pluripotent stem cells. Topics address cell polarity and cell fate; positional information and patterning of development and regeneration; limb, heart and whole body regeneration; stem cell renewal; progenitor cells in development; responses to wounding; and applications of stem cells in development of therapies. Discussions of papers supplement lectures.

Fall 2005 · Developmental Biology

Topics

  • Development and Evolution: Introduction - Prof. Hazel Sive
  • Development and Evolution: Introduction (cont.), Dorsoventral Axis - Prof. Hazel Sive
  • Student Led Discussion Presentation: Vertebrate Development I
  • Dorsoventral Axis (II), Anteroposterior Axis - Prof. Hazel Sive
  • Student Led Discussion Presentation: Vertebrate Development II
  • Nervous System: Background Descriptive, Morphogenesis - Prof. Martha Constantine-Paton
  • Student Led Discussion Presentation: Nervous System I
  • Embryonic Left-Right Asymmetry: A Fundamental Problem of Patterning Bearing on Cell, Developmental, and Evolutionary Biology - Guest Lecturer: Dr. Michael Levin, The Forsyth Institute and Harvard School of Dental Medicine
  • Student Led Discussion Presentation: Left/Right Axis
  • Neural Development: Patterning Cell Types in the Spinal Cord - Prof. Martha Constantine-Paton
  • Student Led Discussion Presentation: Nervous System II
  • Student Led Discussion Presentation: Nervous System III
  • Organogenesis - Guest Lecturer: Dr. Thomas M. Schultheiss, Harvard Medical School
  • Student Led Discussion Presentation: Organogenesis
  • Finding a Morphogen Gradient Using Forward Genetics: Dorsal-ventral Patterning by BMPs in Drosophila Embryos - Guest lecturer: Dr. Laurel Raftery , MGH/Harvard Medical School
  • Student Led Discussion Presentation: Drosophila
  • Morphogenesis: Building 3D Structure - Prof. Hazel Sive
  • Student Led Discussion Presentation: Morphogenesis
  • Stem Cells: Units of Development and Regeneration - Guest lecturer: Dr. Fernando D. Camargo, Whitehead Institute for Biomedical Research
  • Student Led Discussion Presentation: Stem Cells
  • Student Led Discussion Presentation: C. elegans
  • Cloning and Epigenetics - Prof. Hazel Sive
  • Student Led Discussion Presentation: Epigenesis and Cloning
  • Student Led Discussion Presentation: Human Development

Readings

  • Gilbert, Scott F. Developmental Biology . 7th ed. Sunderland, MA: Sinauer Associates, 2003. ISBN: 9780878932580.
    Recommended
  • SES #: 1 · TOPICS: Development and Evolution: Introduction - Prof. Hazel Sive · READINGS: Weaver, C., and D. Kimelman. “Move it or lose it: axis specification in Xenopus.” Development 131, no. 15. (August 2004): 3491-9.
    Reading assignment
  • Yost, C., G. H. Farr, 3rd, S. B. Pierce, D. M. Ferkey, M. M. Chen, and D. Kimelman. “GBP, an inhibitor of GSK-3, is implicated in Xenopus development and oncogenesis.” Cell 93, no. 6 (June 12, 1998): 1031-41.
    Listed reading
  • SES #: 3 · TOPICS: Student Led Discussion Presentation: Vertebrate Development I · READINGS: Kudoh, T., S. W. Wilson, and I. B. Dawid. “Distinct roles for Fgf, Wnt and retinoic acid in posteriorizing the neural ectoderm.” Development 129, no. 18 (September 2002): 4335-46.
    Reading assignment
  • Niehrs, C. “Regionally specific induction by the Spemann-Mangold organizer.” Nat Rev Genet 5, no. 6 (June 2004): 425-34.
    Listed reading
  • SES #: 5 · TOPICS: Student Led Discussion Presentation: Vertebrate Development II · READINGS: Vetter, Monica L. and Richard I. Dorsky. “Neurogenesis.” Chapter 5 in Developmental Neurobiology . Edited by Mahendra S. Rao, and Marcus Jacobson. 4th ed. New York, NY: Springer-Verlag, 2005. ISBN: 9780306483301.
    Reading assignment
  • SES #: 7 · TOPICS: Student Led Discussion Presentation: Nervous System I · READINGS: Grandbarbe, L., J. Bouissac, M. Rand, M. Hrabe de Angelis, S. Artavanis-Tsakonas, and E. D. Mohier. “Delta-Notch signaling controls the generation of neurons/glia from neural stem cells in a stepwise process.” Development 130, no. 7 (April 2003): 1391-402.
    Reading assignment
  • SES #: 9 · TOPICS: Student Led Discussion Presentation: Left/Right Axis · READINGS: Levin, M. “The embryonic origins of left-right asymmetry.” Crit Rev Oral Biol Med 15, no. 4 (July 1, 2004): 197-206.
    Reading assignment
  • Supp, D. M., M. Brueckner, M. R. Kuehn, D. P. Witte, L. A. Lowe, J. McGrath, J. Corrales, and S. S. Potter. “Targeted deletion of the ATP binding domain of left-right dynein confirms its role in specifying development of left-right asymmetries.” Development 126, no. 23 (December 1999): 5495-504.
    Listed reading
  • SES #: 11 · TOPICS: Student Led Discussion Presentation: Nervous System II · READINGS: Dasen, J. S., J. P. Liu, and T. M. Jessel. “Motor neuron columnar fate imposed by sequential phases of Hox-c activity.” Nature 425, no. 6961 (October 30, 2003): 926-33.
    Reading assignment
  • Appel, B., and J. S. Eisen. “Retinoids run rampant: multiple roles during spinal cord and motor neuron development.” Neuron . 40, no. 3 (October 30, 2003): 461-4.
    Listed reading
  • SES #: 12 · TOPICS: Student Led Discussion Presentation: Nervous System III · READINGS: Pasquale, E. B. “Eph receptor signalling casts a wide net on cell behaviour.” Nat Rev Mol Cell Biol 6, no. 6 (June 2005): 462-75.
    Reading assignment
  • Huberman, A. D., K. D. Murray, D. K. Warland, D. A. Feldheim, and B. Chapman. “Ephrin-As mediate targeting of eye-specific projections to the lateral geniculate nucleus.” Nat Neurosci 8, no. 8 (August 2005): 1013-21. (Epub July 17, 2005.)
    Listed reading
  • Charron, F., and M. Tessier-Lavigne. “Novel brain wiring functions for classical morphogens: a role as graded positional cues in axon guidance.” Development 132, no. 10 (May 2005): 2251-62.
    Listed reading
  • Zou, Y., E. Stoeckli, H. Chen, and M. Tessier-Lavigne. “Squeezing axons out of the gray matter: a role for slit and semaphorin proteins from midline and ventral spinal cord.” Cell 102, no. 3 (August 4, 2000): 363-75.
    Listed reading
  • SES #: 14 · TOPICS: Student Led Discussion Presentation: Organogenesis · READINGS: Alsan, B. H., and T. M. Schultheiss. “Regulation of avian cardiogenesis by Fgf8 signaling.” Development 129, no. 8 (April 2002): 1935-43.
    Reading assignment
  • Carroll, T. J., J. S. Park, S. Hayashi, A. Majumdar, and A. P. McMahon. “Wnt9b plays a central role in the regulation of mesenchymal to epithelial transitions underlying organogenesis of the mammalian urogenital system.” Dev Cell 9, no. 2 (August 2005): 283-92.
    Listed reading
  • Dressler, G. “Tubulogenesis in the developing mammalian kidney.” Trends Cell Biol 12, no. 8 (August 2002): 390-5.
    Listed reading
  • Zaffran, S., and M. Frasch. “Early signals in cardiac development.” Circ Res 91, no. 6 (September 20, 2002): 457-69.
    Listed reading
  • SES #: 16 · TOPICS: Student Led Discussion Presentation: Drosophila · READINGS: Blair, S. S. “Lineage compartments in Drosophila.” Curr Biol 13, no. 14 (July 15, 2003): R548-51.
    Reading assignment
  • Gurdon, J. B., and P. Y. Bourillot. “Morphogen gradient interpretation.” Nature 413, no. 6858 (October 25, 2001): 797-803.
    Listed reading
  • Nellen, D., R. Burke, G. Struhl, and K. Basler. “Direct and long-range action of a DPP morphogen gradient.” Cell 85, no. 3 (May 3, 1996): 357-68.
    Listed reading
  • SES #: 18 · TOPICS: Student Led Discussion Presentation: Morphogenesis · READINGS: Wallingford, J. B., R. M. Harland. “Xenopus Dishevelled signaling regulates both neural and mesodermal convergent extension: parallel forces elongating the body axis.” Development 128, no. 13 (July, 2001): 2581-92.
    Reading assignment
  • Strutt, D. “Frizzled signalling and cell polarisation in Drosophila and vertebrates.” Development 130, no. 19 (October 2003): 4501-13.
    Listed reading
  • SES #: 20 · TOPICS: Student Led Discussion Presentation: Stem Cells · READINGS: Fuchs, E., T. Tumbar, and G. Guasch. “Socializing with the neighbors: stem cells and their niche.” Cell 116, no. 6 (March 19, 2004): 769-78.
    Reading assignment
  • Benitah, S. A., M. Frye, M. Glogauer, and F. M. Watt. “Stem cell depletion through epidermal deletion of Rac1.” Science 309, no. 5736 (August 5, 2005): 933-5.
    Listed reading
  • Dotto, G. P., and G. Cotsarelis. “Developmental biology: Rac1 up for epidermal stem cells.” Science 309, no. 5736 (August 5, 2005): 890-1.
    Listed reading
  • SES #: 21 · TOPICS: Student Led Discussion Presentation: C. elegans · READINGS: Ceol, C., and H. R. Horvitz. “A new class of C. elegans synMuv genes implicates a Tip60/NuA4-like HAT complex as a negative regulator of Ras signaling.” Developmental Cell 6 (2004): 563-576.
    Reading assignment
  • Fay D. S., and M. Han. “The synthetic multivulval genes of C. elegans: functional redundancy, Ras-antagonism, and cell fate determination.” Genesis 26, no. 4 (April 2000): 279-84.
    Listed reading
  • SES #: 23 · TOPICS: Student Led Discussion Presentation: Epigenesis and Cloning · READINGS: Gurdon J. B., J. A. Byrne, and S. Simonsson. “Nuclear reprogramming and stem cell creation.” Proc Natl Acad Sci U.S.A. 100 (Suppl 1) (September 30, 2003): 11819-22. (Epub August 14, 2003)
    Reading assignment
  • Nolen, L. D., S. Gao, Z. Han, M. R. Mann, Chung Y. Gie, A. P. Otte, M. S. Bartolomei, and K. E. Latham. “X chromosome reactivation and regulation in cloned embryos.” Dev Biol 279, no. 2 (March 15, 2005): 525-40.
    Listed reading
  • Morgan H. D., F. Santos, K. Green, W. Dean, and W. Reik. “Epigenetic reprogramming in mammals.” Hum Mol Genet 14, spec no. 1 (April 15, 2005): R47-58.
    Listed reading
  • SES #: 24 · TOPICS: Student Led Discussion Presentation: Human Development · READINGS: Vogelstein, B., B. Alberts, and K. Shine. “Genetics. Please don’t call it cloning!” Science 295, no. 5558 (February 15, 2005): 1237.
    Reading assignment
  • Birmingham, Alabama. “Assisted Reproductive Technologies: A Guide for Patients.” American Society for Reproductive Medicine, Patient Information Series. Copyright 2003.
    Listed reading
  • Marx, J. “Neurodegeneration. Huntington’s research points to possible new therapies.” Science 310, no. 5745 (October 7, 2005): 43-5.
    Listed reading
  • Wusthoff, C. “MSJAMA. The dilemma of confidentiality in Huntington disease.” JAMA 290, no. 9 (September 3, 2003): 1219-20.
    Listed reading
  • Sermon, K., A. Van Steirteghem, and I. Lancet Liebaers. “Preimplantation genetic diagnosis.” 363, no. 9421 (May 15, 2004): 1633-41.
    Listed reading
  • Holm, S. “Going to the roots of the stem cell controversy.” Bioethics 16, no. 6 (November 2002): 493-507.
    Listed reading
  • Wolpert, Lewis. Principles of Development. 2nd ed. New York, NY: Oxford University Press, 2001. ISBN: 9780198792918.
    Recommended
  • Kalthoff, Klaus. Analysis of Biological Development . 2nd ed. Boston, MA: McGraw-Hill, 2001. ISBN: 0071180788.
    Recommended
  • Slack, J. M. W. Essential Developmental Biology . Malden, MA: Blackwell Science, 2001. ISBN: 9780632052332.
    Recommended
  • Bier, Ethan. The Coiled Spring: How Life Begins . Plainview, NY: Cold Spring Harbor Laboratory Press, 2000. ISBN 9780879695637.
    Recommended
  • Gerhart, John, and Marc Kirschner. Cells, Embryos, and Evolution: Toward a Cellular and Developmental Understanding of Phenotypic Variation and Evolutionary Adaptability . Malden, MA: Blackwell Science, 1997. ISBN: 9780865425743.
    Recommended
  • Russo, V. E. A., et al., eds. Development: Genetics, Epigenetics, and Environmental Regulation . New York, NY: Springer, 1999. ISBN: 9783540627548.
    Recommended
  • Arias, Alfonso Martinez, and Alison Stewart. Molecular Principles of Animal Development. New York, NY: Oxford University Press, 2002. ISBN: 9780198792840.
    Recommended
  • Rao, Mahendra S., and Marcus Jacobson, eds. Developmental Neurobiology . 4th ed. New York, NY: Springer-Verlag, 2005. ISBN: 9780306483301.
    Recommended

Prerequisites & course information

Prereq: Permission of instructor G (Spring) 4-0-8 units

Sources