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9.12

Experimental Molecular Neurobiology

2-4-6 units. Institute LAB · Undergraduate

Experimental techniques in cellular and molecular neurobiology. Designed for students without previous experience in techniques of cellular and molecular biology. Experimental approaches include DNA manipulation, molecular cloning, protein biochemistry, dissection and culture of brain cells, synaptic protein analysis, immunocytochemistry, and fluorescent microscopy. One lab session plus one paper review session per week. Instruction and practice in written communication provided. Enrollment limited.

Fall 2006

Designed for students without previous experience in techniques of cellular and molecular biology, this class teaches basic experimental techniques in cellular and molecular neurobiology. Experimental approaches covered include tissue culture of neuronal cell lines, dissection and culture of brain cells, DNA manipulation, synaptic protein analysis, immunocytochemistry, and fluorescent microscopy.

Readings

  • Primrose et al. Principles of Gene Manipulation . Oxford, UK: Blackwell Science, 2006. (DNA Recombinant Technology)
    Recommended
  • Alberts et al. Molecular Biology of the Cell . Philadelphia, PA: Taylor and Francis, 2002. (Molecular Biology in general)
    Recommended
  • Martin et al. From Neuron to Brain . Sunderland, MA: Sinauer Associates, Inc., 2000. (Cellular Level Neuroscience in general)
    Recommended
  • WEEK #: 1 · READING ASSIGNMENTS: Introduction
    Reading assignment
  • Julius, David, Amy B. MacDermott, Richard Axel, and Thomas M. Jessel. “Molecular Characterization of a Funcational cDNA Encoding the Serotonin 1c Receptor.” Science 241 (1998): 558-564.
    Listed reading
  • WEEK #: 3 · READING ASSIGNMENTS: Buck, Linda, and Richard Axel. “A Novel Multigene Family May Encode Odorant Receptors: A Molecular Basis for Odor Recognition.” Cell 65 (1991): 175-187.
    Reading assignment
  • WEEK #: 4 · READING ASSIGNMENTS: Question and Answer Session
    Reading assignment
  • WEEK #: 5 · READING ASSIGNMENTS: Shen, Karen, and Tobias Meyer. “Dynamic Control of CaMKII Translocation and Localization in Hippocampal Neurons by NMDA Receptor Stimulation.” Science 284 (1999): 162-166.
    Reading assignment
  • WEEK #: 6 · READING ASSIGNMENTS: Scheiffele, Peter, Jinhong Fan, Jenny Choih, Richard Fetter, and Tito Serafini. “Neuroligin Expressed in Nonneuronal Cells Tiggers Presynaptic Development in Contacting Axons.” Cell 101 (2000): 657-669.
    Reading assignment
  • WEEK #: 7 · READING ASSIGNMENTS: Hua, Jackie Yuanyuan, Matthew C. Smear, Herwig Baler, and Stephen J. Smith. “Regulation of Axon Growth In Vivo by Activity-based Competition.” Nature 434 (2005): 969-973.
    Reading assignment
  • WEEK #: 8 · READING ASSIGNMENTS: Antoch, Marina P., Eun-Joo Song, Anne-Marie Change, Martha Hotz Vitaterna, Yaliang Zhao, Lisa D. Wilsbacher, Ashvin M. Sangoram, David P. King, Lawrence H. Pinto, and Joseph S. Takahashi. “Functional Identification of the Mouse Circadian Clock Gene by Transgenic BAC Rescue.” Cell 89 (1997): 655-667.
    Reading assignment
  • WEEK #: 9 · READING ASSIGNMENTS: Serafini, Tito, Timothy E. Kennedy, Michael J. Galko, Christine Mirzayen, Thomas M. Jessell, and Marie Tessler-Lavigne. “The Netrins Define a Family of Axon Outgrouth-promoting Proteins Homologous to C. elegans UNC-6.” Cell 78 (1994): 409-424.
    Reading assignment
  • Gould, Elizabeth, Alison J. Reeves, Michael S. A. Graziano, and Charles G. Gross. “Neurogenesis in the Neocortex of Adult Primates.” Science 286 (1999): 548-552.
    Listed reading
  • Kornack, David R., and Pasko Rakic. “Cell Proliferations without Neurogenesis in Adult Primate Neocortex.” Science 294 (2001): 2127-2130.
    Listed reading
  • WEEK #: 11 · READING ASSIGNMENTS: MacLauren, R. E., R. A. Pearson, A. MacNeil, R. H. Douglas, T. E. Salt, M. Akimoto, A. Swaroop, J. S. Sowden, and R. R. Ali. “Retinal Repair by Transplantation of Photoreceptor Precursors.” Nature 444 (2006): 203-207.
    Reading assignment
  • WEEK #: 12 · READING ASSIGNMENTS: Davis, Graeme W., and Corey S. Goodman. “Synapse Specific control of Synaptic Efficacy at the Terminals of a Single Neuron.” Nature 392 (1998): 82-86.
    Reading assignment
  • WEEK #: 13 · READING ASSIGNMENTS: Brown, Arthur, Paul A. Yates, Patrick Burrola, Dan Ortuno, Ashish Vaidya, Thomas M. Jessell, Samuel L. Pfaff, Dennis D.M. O’Leary, and Greg Lemke. “Topographic Mapping from the Retina to the Midbrain Is Controlled by Relative but not Absolute Levels of EphA Receptor Signaling.” Cell 102 (2000): 77-88.
    Reading assignment

Prerequisites & course information

Prereq: Biology (GIR) and 9.01 U (Spring) 2-4-6 units. Institute LAB

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