Advanced Cell Biology Lab

(4 Credits)

The course takes an in depth look to understand the fundamental and advanced methods for growing and studying cells—the smallest units of life. This course introduces students to the fundamentals of cell biology and the experimental approaches used in research to examine the cell structure and function. Topics cover cell lines culture, the structure and function of the cells, metabolic pathways, cell signaling pathways, and gene function investigation in vitro in cells. The laboratory course will teach students the skills needed in advanced cellular biology research such as the hands-on techniques of cryopreservation, transfection, realtime PCR, immunofluorescence, RNA isolation, cDNA construction, gene expression and regulation. The lab course will also emphasize literature search, scientific writing, lab notes taking, data analysis, and best practices in lab safety. Prereq for BIOL-SHU 314 is BIO-SHU 21 Foundations of Biology I. Fulfillment: Biology elective; Neural Science Approved upper-level Biology course.