Masters Biotechnology Lab

1 Course Unit

This lab is an introduction to lab techniques in biochemical engineering and biotechnology for graduate students in SEAS. Students are assumed to have a background in cell and molecular biology or to be co-registered for CBE 5540. Labs will include E. coli gene editing with CRISPR, production of eGFP in E. coli cells and isolation by chromatography, a fed-batch bioreactor to grow yeast cells and determine the growth and yield parameters, analysis of immobilized and soluble enzyme kinetics in a stirred reactor. Each lab will include a data analysis assignment. Three individual technical memos will be assigned, and a group report and presentation will be due at the end of the semester. Oral and written communication skills are emphasized to improve technical communication skills. Fall or Spring 1 Course Unit