Methods for in vivo biochemical discovery

0.5 Course Units

The course surveys the chemical machinery of the cell, post-translational modifications of proteins and catalytic machinery of enzymes. Through this survey, students will become familiar with the cellular proteome and its facinating functions and dysfunctions that drive normal physiology and disease states of the cell. Current technologies for in vivo function assignment and unbiased drug-target discovery will be reviewed. Example topics include gene editing, protein profiling, targeted degradation, high-throughput drug screening and quantitative analysis of proteomes/metabolomes from native biological systems. The course couples in-class lecture and discussion with research demonstrations of select methods used for discovery. Fall or Spring 0.5 Course Units