Biological Chemistry of Materials: Principles and Applications

Undergraduate / Graduate

This course will focus on principles of bioconjugation techniques; preparation of immobilized-enzymes/proteins: adsorption, occlusion, cross-linking and covalent binding. Applications of cofactor-dependent enzymes: building of enzymatic electrodes and biofuel cells. Development of immunosensors based on ELISA, electrochemistry, optics, carbon nanotubes and piezoelectric methods. Principles and design of DNA/RNA based sensors (Ribozymes, SELEX, Aptamers, DNAzymes, Molecular Beacons). Amplification methods for nucleic acids detection in test tube and in cells. Preparation and characterization of nanoparticles in nucleic acids and proteins sensing processes.