Molecular Biotechnology Laboratory: From Gene to Product

6 units · Undergraduate upper division (100-199)

Lecture, two hours; laboratory, eight hours; outside study, eight hours. Enforced requisites: courses 101C, C125. Integration of molecular and engineering techniques in modern biotechnology. Cloning of protein-coding gene into plasmid, transformation of construct into E. coli , production of gene product in bioreactor, downstream processing of bioreactor broth to purify recombinant protein, and characterization of purified protein. Letter grading.