3-D Cryo-Electron Microscopy of Macromolecules and Cells

4 credits

The resolution revolution in cryo-electron microscopy has made this a key technology for the high-resolution determination of structures of macromolecular complexes, organelles, and cells. The basic principles of transmission electron microscopy, modern cryo-electron microscopy, image acquisition, and 3-D reconstruction will be discussed. Examples from the research literature using this state-of-the-art technology will also be discussed. Prerequisites: CHEM 114A or BIBC 100.