Laboratory Methods in Synthetic Biology

4 credits

Designs and builds transgenic bacterial using promoters and genes taken from a variety of organisms. Uses construction techniques including recombination, gene synthesis, and gene extraction. Evaluates designs using sequencing, fluorescence assays, enzyme activity assays, and single cell studies using time-lapse microscopy. Prerequisite: E E 423/BIOEN 423/CHEM E 476/CSE 486; and either CHEM 142, CHEM 143, or CHEM 145. Offered: jointly with BIOEN 425/CHEM E 478/CSE 488.