Nonlinear Optical Microscopy

3 units

This course will cover the theoretical and practical knowledge needed to design, construct, and use a nonlinear optical microscope. The course will focus on the relevant optical physics and instrumentation for different types of nonlinear optical microscopy, and additionally provide some information on applications and image processing. Topics include: ultrafast lasers, detectors, nonlinear susceptibility, nonlinear wave equation, quantum theory of nonlinear optics, harmonic generation, multiphoton fluorescence, fluorescence lifetime, optical metabolic imaging, coherent Raman scattering, and multimodal nonlinear optical microscopy.