Integrative Biological Mass Spectrometry

Graduate

This course will focus on the theory and applications of biological mass spectrometry. The most common types of ionization methods and mass analyzers will be thoroughly presented including data interpretation. A major part of the course will be to discuss the applications of this technique in identifying multiprotein complexes, mapping post-translational modifications, structural biology, and quantitative proteomics. Various techniques that are commonly interfaced with the mass spectrometer will be introduced in order to emphasize proper sample preparation and stimulate discussion on applying mass spectrometry to common biological research fields. (2 lecture hours/week.)