everything.study
B M E 651

BIOPHOTONICS LABORATORY

3 credits.

Learn and apply the fundamentals of optical imaging, microscopy and instrumentation via practical hands-on training with a specific emphasis on the life-sciences applications. Topics include constructing imaging systems using fundamental optical tools and instruments, illumination and aberrations, microscopy techniques, resolution and contrast measurement, optical spectroscopy, nanophotonics, bioimaging and biosensing.

Topics

Topics explicitly listed in official catalogue description

Prerequisites & course information

Requisites: PHYSICS 202 , 208 , 248 , MED PHYS/PHYSICS 265 , E C E 320 , 434 , or graduate/professional standing Course Designation: Grad 50% - Counts toward 50% graduate coursework requirement Repeatable for Credit: No Last Taught: Spring 2026 Learning Outcomes: 1. Identify and use optical tools and instruments and construct optical imaging systems Audience: Both Grad & Undergrad 2. Measure optical resolution and summarize parameters, such as optical aberrations, illumination and collecting optical system settings, that affect resolution in optical imaging Audience: Both Grad & Undergrad 3. Implement Kohler illumination, identify conjugate planes and illustrate Abbe theory of image formation on a hands-on optical hardware Audience: Both Grad & Undergrad 4. Measure contrast in different microscopy techniques and identify parameters that contribute to signal-to-background Audience: Both Grad & Undergrad 5. Measure and analyze light spectra using filters and spectrometers to identify different light sources, and nanoparticle and light interactions Audience: Both Grad & Undergrad 6. Follow and implement written experiment protocols, collect, and analyze data, and write coherent reports presenting experimental findings Audience: Both Grad & Undergrad 7. Identify and evaluate an optical imaging setup that was developed in a recently published journal article applying knowledge that was learned in class Audience: Graduate Learning Outcomes: 1. Identify and use optical tools and instruments and construct optical imaging systems Audience: Both Grad & Undergrad 2. Measure optical resolution and summarize parameters, such as optical aberrations, illumination and collecting optical system settings, that affect resolution in optical imaging Audience: Both Grad & Undergrad 3. Implement Kohler illumination, identify conjugate planes and illustrate Abbe theory of image formation on a hands-on optical hardware Audience: Both Grad & Undergrad 4. Measure contrast in different microscopy techniques and identify parameters that contribute to signal-to-background Audience: Both Grad & Undergrad 5. Measure and analyze light spectra using filters and spectrometers to identify different light sources, and nanoparticle and light interactions Audience: Both Grad & Undergrad 6. Follow and implement written experiment protocols, collect, and analyze data, and write coherent reports presenting experimental findings Audience: Both Grad & Undergrad 7. Identify and evaluate an optical imaging setup that was developed in a recently published journal article applying knowledge that was learned in class Audience: Graduate

Sources