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ONCOLOGY 673

PURIFICATION AND CHARACTERIZATION OF PROTEIN AND PROTEIN COMPLEXES

2 credits.

The theory and practice of protein purification. Topics covered include conventional and recent protein fractionation techniques; enzyme assays, handling, and characterization; purification strategy; and overproduction of cloned gene products. The emphasis is on micro and laboratory scale purifications.

Topics

Topics explicitly listed in official catalogue description

Prerequisites & course information

Requisites: BIOCHEM 508 , CHEM 511 or graduate/professional standing Course Designation: Core GenEd - Natural Science & Wellness Grad 50% - Counts toward 50% graduate coursework requirement Repeatable for Credit: No Last Taught: Spring 2022 Learning Outcomes: 1. Demonstrate fundamental knowledge and understanding foundational concepts in the areas of protein purification, purification of recombinant proteins, and protein characterization and use. Audience: Both Grad & Undergrad 2. Read and effectively evaluate current literature. Audience: Both Grad & Undergrad 3. Actively engage in the latest scholarly research discussions. Audience: Graduate Learning Outcomes: 1. Demonstrate fundamental knowledge and understanding foundational concepts in the areas of protein purification, purification of recombinant proteins, and protein characterization and use. Audience: Both Grad & Undergrad 2. Read and effectively evaluate current literature. Audience: Both Grad & Undergrad 3. Actively engage in the latest scholarly research discussions. Audience: Graduate

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