Quantitative Biochemical Imaging

Graduate / Professional

Theory of optical microscopy, imaging, and image analysis with emphasis on quantitative characterization of the structure, dynamics, and chemical reactions of proteins, nucleic acids, and other biopolymers. Topics include optics of microscope and image formation, interaction of light and matter, fluorescent probes and biosensors, digital image processing, modern approaches in light microscopy (including confocal and multiphoton), and a brief introduction to electron microscopy and scanning probe techniques. Prerequisite: physical chemistry that includes quantum mechanics; calculus and linear algebra are recommended but not required. ½ Course cr TTh 9am-10:15am