Light Microscopy: Techniques and Image Analysis

Graduate / Professional

A rigorous study of principles and pertinent modalities involved in modern light microscopy. The overall course learning objective is to develop competencies involving advanced light microscopy applications common to multidisciplinary research. Laboratory modules coupled with critical analysis of pertinent research papers cover all major light microscope methods—from the basics (principles of optics, image contrast, detector types, fluorescence, 1P and 2P excitation, widefield, confocal principle, TIRF), to more recent advances, including super-resolution, light sheet, FLIM/FRET, motion analysis and force measurements. Enrollment is capped at eight to promote interactions and ensure a favorable hands-on experience. Priority is given to students who are planning on using these techniques in their independent research. MW 1:30pm-4:30pm